Cyclophosphamide treatment evoked side effect on skeletal muscle actin, monitored by DSC

Abstract Several kind of drugs—used in cancer treatments—such as cyclophosphamide (CP) can also trigger a disease classified toxic polyneuropathy. Polyneuropathy is simultaneous malfunction several peripheral nerves, typical side effect therapy. In our previous study, we used CP treated vitro animal model (Guinea pig) with comparable dosage and time handling human protocol to show evidences this drug-induced effects. We could dose-dependent difference between T m ?H cal untreated samples assigned their intact muscle nerve, blood plasma red cells. current study analyze on skeletal actin (prepared from m. psoas rabbit) by DSC (differential scanning calorimetry), follow the possible consequence drug treatment “activator” contraction. have demonstrated that run curves, s together exhibit clear effect. case Ca 2+ G it manifested well separated second high denaturing temperature binding into cleft. This way nucleotide cleft subdomains 1 3 becomes less flexible, indicating sensitivity treatment. F-actin samples, main peak represents thermal denaturation 3, increased calorimetric enthalpy administrating refers more rigid structure. These alterations be molecular background polyneuropathy after